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USDA-ARS, Plant Sciences Institute, Soybean Genomics and Improvement Lab., 10300 Baltimore Ave, Bldg. 006, Beltsville, MD 20705
* Corresponding author (ben.matthews{at}ars.usda.gov).
MiniMax soybean [Glycine max (L.) Merr.] (Reg. No. CV-489, PI 643148) was developed by the USDA-ARS at Beltsville, MD. It was released on April 13, 2006 because of its small stature, early maturity date, and small seed size, to allow more plants to be grown and to mature in a smaller space than other soybean cultivars (Fig. 1 ). It is intended for researchers with limited space who desire high-density planting and as a basic laboratory model for soybean genomics, biochemical, plant disease and physiological studies.
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MiniMax was planted in the field June first at Beltsville, MD (approx. 39° North latitude) matured in 73 to 85 d with an average plant height of 22 cm. Plants were also grown in the greenhouse in 16 h light/8 h dark for the first 16 d, then at 12 h light/12h dark to initiate flowering, which began at 23 to 27 d. The temperature was maintained between 72 and 80o F during the day and 72 to 75o F at night. Natural greenhouse lighting was supplemented with sodium vapor lights during winter months. Dry brown pods can be harvested at 9 to 11 wk after planting. In the greenhouse the small seed averaged about 5.1 g/100–1 seeds and 14 seeds per plant in the summer. MiniMax has purple flowers, brown pods, gray pubescence and produces seeds with a yellow seed coat and hilum. Mature seeds of MiniMax had 42.19% protein and 12.10% oil. It is susceptible to soybean cyst nematode (Heterodera glycines) HG Type 0 (Race 3; FI 59%), HG Type 1.2.5.7 (Race 2; FI 92%), HG Type 2.5.7 (Race 5; FI 70%), and is moderately resistant to HG Type 2.5.7 (Race 1; FI 30%) (Dr. P. Rao Arelli, personal communication, 2006). The HG Type classification is based on the system proposed by Niblack et al. (2002).
MiniMax as well as the AC Colombe, T263 and Nattosan parents were characterized with a core set of 13 simple sequence repeat (SSR) loci (Table 1 ). These SSR loci were from different linkage groups and were determined to be effective in soybean DNA fingerprinting (Song et al., 1999). These 13 marker loci provide unique allelic fingerprints of even closely related soybean cultivars. The allele sizes of 13 loci genotyped in MiniMax and its parents are listed in Table 1. The allele size of each locus from MiniMax was compared with those from 402 genotypes including 35 ancestors of North American cultivars, 66 North American elite soybean cultivars, 21 Korean elite genotypes, 30 Japanese elite genotypes, 59 Chinese elite cultivars, and 170 plant introductions from China, Korea, and Japan. Allele sizes were determined on the Applied Biosystems 3730 DNA Analyzer (University Park, IL). A 220 bp allele at the Satt009 locus is unique to MiniMax and is apparently the result of a mutation. The average genetic distance of MiniMax with 402 genotypes was 0.80, and the closest distance to any other genotype was 0.50. The allelic profile of MiniMax at the 13 SSR loci was unique from all 402 genotypes in the allele size database.
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Footnotes
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Received for publication October 12, 2006.
References
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